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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Potato producing vaccine against cholera
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potato (Solanum tuberosum) 35SctxBSEK
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Organization:University of Rostock (), Agrobiotechnology - Faculty of Agricultural and Environmental SciencesFaculty of Agricultural and Environmental Sciences Justus-von-Liebig-Weg 6Rostock,
18059,Phone: +49 381 498 3001,Fax:Email: dekan.auf@uni-rostock.de,
Expression of the non-toxic B subunit of the cholera toxin . The non-toxic B subunit has been used for more than 20 years as a component of human vaccines against cholera and other diarrheal diseases.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
Cultivar/ breeding line: desiree, albatros
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p35SctxBSEK, derivative of pLH9000
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-102896-4 Cholera Toxin | Vibrio choleraeProtein coding sequence | Production of medical or pharmaceutical compounds (human or animal) (Vaccines)
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BCH-GENE-SCBD-102033-4 SEKDEL ER retention signalTransit signal
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
The LMO 35SctxBSEK expresses a synthetic gene of the nontoxic subunit of cholera toxin that corresponds to 71% with the gene sequence of the ctxB gene from Vibrio cholerae (100% amino acid identity). The gene was adapted to the codon preference of higher plants. The ER-retention signal SEKDEL was fused to the cholera toxin subunit in order to stabilize the protein.
In the plant, expression is regulated by the 35S promoter and terminator from the cauliflower mosaic virus, which leads to an expression in all plant parts throughout the entire lifecycle of the potato.
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In the plant, expression is regulated by the 35S promoter and terminator from the cauliflower mosaic virus, which leads to an expression in all plant parts throughout the entire lifecycle of the potato.
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- Research
PCR-based methods, to be developed on the information about the transformation vector.
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| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
